Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIK-GKDVALAKGEYSNLKITTVTDLKIAKSMIEKD 5DDT Chain:B ((2-225)) SYDVVIPAAGQGKRMKA-GRNKLFIELKGDPVIIHTLRVFDSHRQCDKIILVINEQEREHFQQLLSDY-PFQTS-IELVAGGDERQHSVYKGLKAVK------QEKIVLVHDGARPFIKHEQIDELIAEAEQTGAAILAVPVKDTIKRVQ-DLQVSETIERSSLWAVQTPQAFRLSLLMKAHAEAE-RKGFLGTDDASLVEQMEGGSVRVVEGSYTNIKLTTPDDLTSAEAIMES-

General information: TITO was launched using: RESULT: Template: 5DDT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1155 -102832 -89.03 -461.13 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -89.03 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_5DDT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DDT-query.scw PDB file : Tito_Scwrl_5DDT.pdb: