Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKGGQMKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQISVGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKP-DRDFEILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDSQGKIGKIQFGAISNADAEAAFKEMN 4NMU Chain:C ((7-145)) ----------------------------------------------EKMQIGKEAPNFVVTDLEGKKIELKDLKGKGVFLNFWGTWCKPCEKEMPYMNELYPKYKEKGVEIIALDA--D---ETDIAVKNFVNQYGL-KFPVAIDKGQKIIGTYGVGPLPTSFLIDKDGKVVEQIIGEQTKEQLEGYLKKI-

General information: TITO was launched using: RESULT: Template: 4NMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 657 -35766 -54.44 -259.17 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -54.44 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_4NMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NMU-query.scw PDB file : Tito_Scwrl_4NMU.pdb: