Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWVKDMFILGSGDQGLAVLSIYEG-------EVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDNFKREEIFCSYK-NKVEYINVICNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGSIVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRDA-----VSIGFGAIIGAGATVIHNVPENAVVVGTPGKIIKYRSV 4M9C Chain:C ((2-215)) --TMIIGVYGASGFGKEVMPLVRQQFPTLSKEQFAFIDDGLS----GTTLNGYPVLSYLDFISKP-A---DHKAVTIAIANSVVREKLVSLLEKDGVQHLAVQSTNTVILDEVEIGEGSLLCPFTCLTSNIKIGKFFHANIYSYVAHDCVIGDYVTFAPGAKCNGNIHIEDHAYIGTGAVIKQGTPDKPLIIGKGAIVGMGAVVTKSVPAGVTVVGNPARILER---

General information: TITO was launched using: RESULT: Template: 4M9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1150 -50224 -43.67 -249.87 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -43.67 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_4M9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M9C-query.scw PDB file : Tito_Scwrl_4M9C.pdb: