Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNEMMHRPVVKEELLDFMRTQQK--KLPGELGKLEEEAHVA--EVPVIPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKAKKNYERLGLTDKVTLLEGQAADILPEL------SGPYDFIFMDSAKSKYIEFLPECLRLLRKGGVLMVDDIFQGGTILLPDEEI-PRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILMTKESETIEL 5KVA Chain:B ((56-278)) ----------KSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKIDFREGPALPVLDDLIADEKNHGSFDFVFVDADKDNYLNYHDRLLKLVKLGGLIGYDNTLWNGSVVLPDDAPMRKYIRFYRDFVLVLNKALAADERVEICQLPVGDGVTLCRRVK-----

General information: TITO was launched using: RESULT: Template: 5KVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 972 14519 14.94 68.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : 14.94 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_5KVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KVA-query.scw PDB file : Tito_Scwrl_5KVA.pdb: