Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLTSRTAQAAHAQNDVAAQRPPPPLYPSMNVGVSAVHTHQNRPCHPYDSQPAWQHPQLGTAPPAVIAGASLAPEQQEQAPQPQQPQQTHQQGRSNQPQSIFGGRFTLLDRLGSGGFGEVYRAEERDQLVPIAVKVERVTDTNALPEQSFLFHEAKVMQEIHKSIQAYMAAQQQHQLMLLQQEKARSGNGRRAENEAAAAADETRQEKVGIAKLKYYGQDGMSRVLIMSLHGQSVANVHRHQGR-LSLFATVMIADQVLRSLEHVHRAGYVHADLKPDNILFGREDPEQL-YLVDFGLSVHFRD-RKGKHRPLITNHSFVGTPRYASLRTHMGHTLSRRDDIEQLVYVMIYLFRGRLPWSGLRISDPDAKEKRIAQMKAEMTLDSICAGCPEAFRDVLNYARCMEFEEEPQYQFLHVLLCSLRDSCTELSSDPNGVPANGSGGVMPQMLTLNANCSTRQHPKAQNGGDAVANGGAVCMTAAEAGLVKNTNIASGVVEQIDGVAENPMMMSSRGTGAPAFFSDAIGQGFLSGNGTDAGPLSPRIDCLNGQPFSPPPLDLQRPCGSPQLRQMR 3UZP Chain:A ((8-278)) ------------------------------------------------------------------------------------------------------GNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHP---------QLHIESKIYKMMQG----------------------------------------GVGIPTIRWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNL-----------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UZP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1284 -55631 -43.33 -207.58 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -43.33 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_3UZP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UZP-query.scw PDB file : Tito_Scwrl_3UZP.pdb: