Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEANMKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSEKVTTVAEFGDSSKLTVGETAIAIGSPLG-SEYANT--VTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDI-RRLNIPSNVTSGVIVRSVQSNMPAN-GHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES 3PV3 Chain:A ((73-347)) ----------------------------------------------------------------------------------------------------RKFESIGSGVIIDPNN--GVIITNDHVIRNASLITVTLQDGRRLKARLIGGDSETDLAVLKIDAKNLKSLV-IGDSDKLEVGDFVVAIGNPFGLN----SQSATFGIVSALKRS-D------ENF----IQTDAAINPGNAGGALVNAKGELIGINTAILS-----V--GIGFAIPINMVKDVAQQIIKFGSIHRGLMGIFVQHL----TPELAQAMGYPEDF-QGALVSQVNPNSPAELAGLKAGDIITQINDTKITQATQVKTTISLLRVGSTVKIIVERDNKPLTLS------------

General information: TITO was launched using: RESULT: Template: 3PV3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1347 -12424 -9.22 -48.72 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -9.22 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_3PV3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PV3-query.scw PDB file : Tito_Scwrl_3PV3.pdb: