Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MQNQTASNLPSTQLGKALL--CLMTS-------ALLFSIMGVCIRFASQTVDNATVVFFRNAVGLFIFIPMLFKQGLDFI-KTDKLWMHTWRSIVGLA-------------AMYGFFYAIANLKLSNAMVFSYSSPIFIPLIAWLFLKEKITKSMIFAAVI----------------------GLIGVL----------FVAKP---DQGLFNALSFIGLGACFLSAMAFVTVRALTSTEPPERIVFYFCVFGSLISSIPMFWHWRIFTWHELVLLIAAGLLANISQLFMSYAY----SLAPAGQIGPMNYIAIIFA---GIWG-------------------FVFWHELPDLFSIIGIFIILFAILLCNPFLQKKLLSRFK-- 1MKF Chain:A ((12-382)) HSSGVSTQSV-DLSQIKRGDEIQAHCLTPAETEVTECAGILKDVLSKNLHELQGLCNVKNKMGVPWVSVEELGQEIITGRLPFPSVGGTPVNDLVRVLVVAESNTPEETPEEEFYAYVELQTELYTFGLSDDNVVFTSD--YMTVWMIDIPKSYVDVGMLTRATFLEQWPGAKVTVMIPYSSTFTWCGELGAISEESAPQPSLSARSPVCKNSARYSTSKFCEVDGCTAETGMEKMSLLTPFGGPPQQAKMNTCPCYYKYSVSPLP------AMDHLILADLAGLDSLTSPVYVMAAYFDSTHENPVRPSSKLYHCALQMTSHDGVWTSTSSEQCPIRLVEGQSQNVLQVRVAPTSMPNLVGVSLMLEG--------QQYRLEYFGDH

General information: TITO was launched using: RESULT: Template: 1MKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1332 -92019 -69.08 -327.47 target 2D structure prediction score : 0.19 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -69.08 2D Compatibility (Sec. Struct. Predict.) : 0.19 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_1MKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MKF-query.scw PDB file : Tito_Scwrl_1MKF.pdb: