Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKQPYTPGEFQWSFLLPKYWGVWIAITFLMLLAILPWAIQWRLAHGLANLAWKYLKSRRKTTIRNLEVCFPEWSPEKVQQQAKQVFVDMMLGIFETLNAWYKPYWFKNRVTIEGLEHITNAQAQGKGVLLLGTHSTLLDAGGYVCAQYFEPDVVYRPQNNP---LLDMLIYRCR---GTIYKAQIDHDDMRGLIRHLKEGDAIWYSPDQDFGLKQGVMAPFFGVPAATVTAHRRLLKISKAVAVPLYFYRHGNVQNPKYHILIEPAVDNLPSEDEVDDATRVNKIIENQLRIAPTQYMWFHRRFKTRPEGYEEIY 5F2T Chain:A ((44-290)) --------GGPEQLRRNLARVVGK------------PPADVPDDLIRASLASYARYWREAFRLPAMDHGRLGEQ-------------------------------------LDVIDIDHLWSALDAGRGAVLALPHSGNWDMAGVWLVQNYGPFTTVAERLKPESLYRRFVEYRESLGFEVLPLTGGERPPFEVLAERLTDNRPICLMAERDLT-RSGVQVDFFGEATRMPAGPAKLAIETGAALFPVHCWFEGD----GWGMRVYPELDT-SSGDVTAITQALADRFAANIATYPADWHMLQPQWIADL-------

General information: TITO was launched using: RESULT: Template: 5F2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1181 -10113 -8.56 -41.96 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -8.56 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_5F2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F2T-query.scw PDB file : Tito_Scwrl_5F2T.pdb: