Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEEPSPSWSMRGLRKWLGTAPETRDELLKLVQNSRRFLEPDTVAMLEGVLDLPATKIREVMTPRTAMISMQEDDQLLD--ILHVLVESAHSRFPVFSADQPDNVVGILLAKDLLPFLTEPNTKLD-IGSLMRQPL-FVPESARSDQVLRMLKHTQTHIAIVI--------DEYGSTAGLVTLEDILEEIV-GEIEDEHDTADEDAQYIVPDNDHTIANAWMVQALTPIEHFNTVLDADFSDDEVETVGGLLLQEIGLVSDLQGQTVELGNWLFTIVEADARTIHLIRAVRQ 5TSR Chain:B ((5-149)) ---------------------------------------------MIQGVLELRCRTVEDVLTPLEDCF-MLDASTVLDFGVLASIMQSGHTRIPVYEEER-SNIVDMLYLKDLAFVDPEDCTPLSTITRFYNHPLHFVFNDTKLDAVLEEFKRGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIR----------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TSR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 480 -27833 -57.99 -210.86 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -57.99 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_5TSR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TSR-query.scw PDB file : Tito_Scwrl_5TSR.pdb: