Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHYEIVLLVHPD-QSDQVVGMVERYISQIKE-ADGQIHRLEDWGRRQLAYPINKIHKAHYILMNVECGQSTLDELEELFRYNDAIIRNLIIRREHAITEESLLAKSAEEKRARKAQREEAQQVAQEAE 1VMB Chain:A ((19-123)) -RIYESMFIIAPNVPEEERENLVERVKKIIEERVKGKIDKVERMGMRKFAYEIKKFNEGDYTVIYFRCDGQNLQELENFYRVTPEIIRWQTFRRFDL------------EKKERKAQR-----------

General information: TITO was launched using: RESULT: Template: 1VMB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 411 -8090 -19.68 -78.54 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -19.68 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.648

(partial model without unconserved sides chains): PDB file : Tito_1VMB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VMB-query.scw PDB file : Tito_Scwrl_1VMB.pdb: