Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLKIKAGFDPTAPDLHLGHTVLINKLKTFQDLGHEVTFLIGDYTAMIGDPTGKSATRPPLSREQVEANAKTYQEQVFKIL----DPNKTKVRFNSEWFNQKSAADLIQ-LASQQTVSRMLERDDFTKRYNNHQPIAIHEFLYPLVQGYDSIALE----ADVELGGTDQTFNLLMGRTL-QSRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGV---FDAPGAMYQKVLSMPDSLIERYFDLLSFKSLDEIKALLDEIAAGRNPQEVKRILALELVERFHDAEAAANAHKSAGNRITEGEVPADTPEVTISRGEFGGEIFIATILRVAGLNPNAAAAKDAVARGAVKVDWNVVDASFSVKENGTFIIQSGKKAIARVTFTD 1TYC Chain:A ((7-315)) ------------LQWRGLVNQTTDEDGLRKLLNEERVTLYCGFDPTADSLHIGHLAPILTMRRFQQAGHRPIALVGGATGLIGDPSGKKSERTLNAKETVEAWSARIKEQLGRFLDFEADGNPAKIKNNYDWIGPLDVITFLRDVGKHFSVNYMMAKESVQSRIET--GISFTEFSYMMLQAYDFLRLYETEGCRLQIGGSDQWGNITAGLELIRKTKG--RAFGLTIPLVTKADGT-KFGKTESGTIWLDKEKTSPYEFYQFWINTDDRDVIRYLKYFTFLSKEEIEALEQELREAPEKRAAQKTLAEEVTKLVHGEEALRQA---------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TYC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1414 -6407 -4.53 -21.79 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -4.53 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1TYC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TYC-query.scw PDB file : Tito_Scwrl_1TYC.pdb: