Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANLKEIRAKVASIKSTQKITRAMQMVAASKMRRAQERMAQGRPYADNMRRVIAHLVQANPEYKHRYMVDRPVKRVGYIIVSSDRGLAGGLNINLFKKVVQHVKAQQEQSIEVQFALIGQKAVSFF-KNYGGKVLGATTQIGDAPSLEQLTGSVQVMLDAFDKG-ELDRIYLVSNGFVNAMTQKPKVEQLVPLAPAEEGDDLNRTYGWDYIYEPEAEELLNGLLVRYIESMVYQGVIENVACEQSARMVAMKAATDNAGQLIKDLQLIYNKLRQAAITQEISEIVGGAAAV 2W6H Chain:G ((1-272)) -ATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARVYGVGSLALYEKADIKTPE-----LI---------IGVSSDRGLCGAIHSSVAKQMKSEAANL----KEVKIIGVGDKIRSILHRTHSDQFLVTFKEVGRRPP--TFGDASVIALELLNSGYEFDEGSIIFNRFRSVISYKTEEKPIFSLDTISSAESMS-------IYDDIDADVLRNYQEYSLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAAL

General information: TITO was launched using: RESULT: Template: 2W6H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1100 -25924 -23.57 -99.33 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain G : 0.77 3D Compatibility (PKB) : -23.57 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2W6H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W6H-query.scw PDB file : Tito_Scwrl_2W6H.pdb: