Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDILENPLELCGFAFIEFVSK--ENELDPIFETIGFSKVAK------HKSKKAYLWRQGNINIILNY------QPESYASFFFNEHGPSACAMGFKTRDAAKAFKKAVELGA----EPMYSKVGPMELNIPAIKGIGGMPIFLVDCDI----YENDFVFFDDAQRNPEGAGLKEIDHLTHNVYKGRMEYWANFYEKIFNFQEIRYFDIKGE---YTGLTSKALTAPDGMIRIPLNE-DSDKGNGQIAEFLADFNGEGIQHIAFICDDLISTWDKLKGLGLKFMTPPPNTYYEMLEERLPEHGEPTEELKQRCILLDGSTKDGQKKLLLQIFSENMI--GPVFFEFIQRKDDDGFGEGNFKALFESIERDQIRRGVLEAK 1T47 Chain:A ((16-377)) -----DPFPVKGMDAVVFAVGNAKQAAHYYSTAFGMQLVAYSGPENGSRETASYVLTNGSARFVLTSVIKPATPWGHFLADHVAEHGDGVVDLAIEVPDARAAHAYAIEHGARSVAEPYELKDEHGTVVLAAIATYGKTRHTLVDRTGYDGPYLPGYVAAAPIVEPPAHRTFQAIDHCVGNVELGRMNEWVGFYNKVMGFTNMKEFVGDDIATEYSALMSKVVADGTLKVKFPINEPALAKKKSQIDEYLEFYGGAGVQHIALNTGDIVETVRTMRAAGVQFL-DTPDSYYDTLGEWVGDTRVPVDTLRELKILADRDEDG----YLLQIFTKPVQDRPTVFFEIIERHGSMGFGKGNFKALFEAIEREQEK-------

General information: TITO was launched using: RESULT: Template: 1T47.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1870 18083 9.67 54.14 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 9.67 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_1T47.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T47-query.scw PDB file : Tito_Scwrl_1T47.pdb: