Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTRHFSQLPKAALAYPKVVQGLIFKKPKGPKILPQVEYVVDTLEIDQNHLKAYNEVCGFQNNGFVPAIYLAVLSQSLQMHMMTAEAFPFPILGLVHIRNQIKQTRPIGVTEKLTLSCKLGELKPHDKGVQFDFITTAKVGNEVVMEGLTTYLSRQKVEKRVGEKAKEEQAPAYVPKAEWNILENTGRRYAKVSGDFNLIHIHAITAKAFGFKQAIAHGMWSKAKALANLELPNAYEADVWFKLPMFLPSKVEFLTANADKKTDFLIRNAKSQKPHVAGTVKAL 4RV2 Chain:B ((29-57)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YAGVSGDLNPIHWDDEIAKQVGLDTAIAH------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 63 -9440 -149.84 -325.52 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -149.84 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_4RV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RV2-query.scw PDB file : Tito_Scwrl_4RV2.pdb: