Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQYQPPHINRKLNVQAWWLTALLISINVGLFIWQIISGVDISDPAIKDALHWGADFTPLTFSGQPERLFTSMFFHFGIIHLLLNMWALYIFGNVAEALFGRLYFIGLYLLAGLFGSLLSSYINIQNGHELLQHFDQSLLPHVSAGASGAVMGLGAALTVLSLFPPLPHQAYILDKKALLMIMAINLI---FGFVATGINNAAHVGGMIMGALLALIWYLSYRTPFKSLLKFLGLVGGLFMTVGFYVYCNQLNSPLLPLWHEVLIQNPEILP 2IC8 Chain:A ((37-177)) --------------------------------------------------------FDP-TLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGS----GKLIVITLISALLSGYVQ--------QKFSGPWF----GGLSGVVYALMGYVWLRGERDP---QSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFV-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2IC8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 590 -98575 -167.08 -714.31 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -167.08 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_2IC8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IC8-query.scw PDB file : Tito_Scwrl_2IC8.pdb: