Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPISNQDLRKAGLKVTLPRIKILELLENSKQHHLSAEDIYKTLLEQGEDVGLATVYRVLTQFEAAGIIQRHHFENNHSVFEIMQEDHHDHLVCQNCNKVIEFTNDVIEKEQHSVAEQHGFTLTGHSLNLYGYCNEPECQEALRKK 2FE3 Chain:A ((11-138)) -----ETLKETGVRITPQRHAILEYLVNSMAH-PTADDIYKALEGKFPNMSVATVYNNLRVFRESGLVKELTYGDASSRFDFVTSDHY-HAICENCGKIVDFHYPGLDEVEQLAAHVTGFKVSHHRLEIYGVCQE----------

General information: TITO was launched using: RESULT: Template: 2FE3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 404 -70926 -175.56 -554.11 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -175.56 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_2FE3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FE3-query.scw PDB file : Tito_Scwrl_2FE3.pdb: