Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRRLSTQDQNFKQVFAELLAFETVNDPNLVQTVDQIIADVRQHGDDHVLKLTQQFDRHPAHQFSDLELSQQQLKAAFDGLNQEIREALQLAADRIRTFHEAQKQDSWTYVDDLGNTLGQKVTPLDRVGIYVPGGLASYPSSVLMNAVPAHVAGVPEIIMVVPAPNGELNPLVLAAAYLAGVSRVFTIGGAQAVAALAYGTQTVPRVDKITGPGNRFVAAAKRAVFGQLGIDMIAGPSEILVYAEGVNNAEWLAMDVLSQAEHDTVAQAIFITPDGALLEAVESAIEKHLNELPKAEIARTSIENRGALVLVKDRAEAVDLINQVAPEHLELCLDDAEAMSQDIRHAGAIFMGRYTPEAIGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSLIMCSKDGVKTLAKTADVLAVQENLDAHARSARYRYQ 4GIC Chain:B ((6-403)) KIKRLYTGDADFASQLDRLLAWS---DTDIHQRVTEIIGCIRRDGDAALVELTARFDHFVVDTAAALELPRDVLEAAWQALPAEQAKALREAAERIRAYAERQKLDSWDYREADGTLLGQKITPLDRVGLYVPGGKAAYPSSVLMNAVPAKVAGVPELIMAVPAPRGELNALVLAAAYISGVDRVFRIGGAQAVAALAYGTETVPRVDKIVGPGNIYVATAKKLVFGQVGIDMVAGPSEILVISDGRTDPDWIAMDLFSQAEHDEDAQAILISPDAAHLEAVQASIERLLPGMERAEVIRTSLERRGGMILVDDLEQAAAVANRIAPEHLELSVESPEVLVESIRNAGAIFMG----------------VLPTSGTARFSSPLGVYDFQKRSSLIYCS--------------------------------

General information: TITO was launched using: RESULT: Template: 4GIC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2050 -249311 -121.62 -657.81 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -121.62 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_4GIC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GIC-query.scw PDB file : Tito_Scwrl_4GIC.pdb: