Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPAVLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPY-LYGLGLVVMGLLLKFYDQNLAASTGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIVTIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTTEGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSNIYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLIMMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIG---IGLILSMRYQNEAPARSSRR------------ 1L9V Chain:A ((1-313)) --------------------------MAELACFCYPHLENDS----------YKFIPFNNLAIKAMLTAKVDKKDMDKFYDSIIYGIAPPPQFKKRYNTN----------DNSRGMNFETIMFTKVAMLICEALNSLKVTQANVSNVLSR--------VVSIRHLENLVIRKENPQDILFH-------------SKDLLLKSTLIAIGQSKEIETTITAEGGEIVFQNAAFTMWKLTYLEHQLMPILDQNFIEYKVTLN--------------------EDKPISDVHVKELVAELRWQYNKFAVITHGKGHYRIVK-----------YSSVANHADRVYATFKSNVKTGVNNDFNLLDQRIIWQNWYAFTSSMKQGNTLDVCKRLLFQKMKPEKNPFKGLSTDRKMDEVS

General information: TITO was launched using: RESULT: Template: 1L9V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1399 -132411 -94.65 -445.83 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -94.65 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_1L9V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L9V-query.scw PDB file : Tito_Scwrl_1L9V.pdb: