Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVETAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVHQDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEVADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAVEVTDEKAFFRLTKAAFQQRRKTLWNNLQHS-----YGKDDQTKAWLAKSLETAGIDPKRRGETLSLQEFAALSNAMSENMQ 3FYD Chain:A ((1-221)) -----------------------------QCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEIDKSLEPYANKLKELYNNIEIIWGDALKVDLN---KLDFN-----KVVANLPYQISSPITFKLIKRGF--DLAVLMYQYEFAKRMVAAAGTKDYGRLSVAVQSRADVEIVAKVPPSAFYPKPKVYSAIVKI-KPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKK--------------------------------------

General information: TITO was launched using: RESULT: Template: 3FYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1035 -133797 -129.27 -619.43 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -129.27 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_3FYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FYD-query.scw PDB file : Tito_Scwrl_3FYD.pdb: