Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MICSANSERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVH------VFHAP--ERNFYNLEKLWSDAP-----LVD--LSEWLD-------- 5HBQ Chain:A ((22-124)) TTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLSVLEQSGLPAKLRHEQIIFHDQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLPVAVN

General information: TITO was launched using: RESULT: Template: 5HBQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 273 691 2.53 8.63 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 2.53 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.190

(partial model without unconserved sides chains): PDB file : Tito_5HBQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HBQ-query.scw PDB file : Tito_Scwrl_5HBQ.pdb: