Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------NTCKCAGKASDQSAINDITKACCVTASYSGKVLEGTYKSDGTCDYSNNSQFKSLSADAATSGFSA---CCKGTTFKEGSSATGGSCSK- 3ZNU Chain:A ((1-94)) MLYLVRMTVNLPRNLDPREEERLKASEKARSRTLQEQGQ-WRYLWRTTG--KYGNISVFDVNSHDELHEILWSLPFFPYLTIDVEPLSHHPARVGKD

General information: TITO was launched using: RESULT: Template: 3ZNU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 272 18274 67.18 222.85 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 67.18 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_3ZNU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZNU-query.scw PDB file : Tito_Scwrl_3ZNU.pdb: