Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --WTGKSEASNRPPPGRRPEETTWFTSDPSYACGVKIFRPGSEGSSGLAEITHLNFHEKIK-----V-LKGQYECEVGPSCSTLR----CTMPRGWRLDIFGKSFPTRRTTYFAENGNPEYNTLAS-EANIPLYD---------------CRVVIRFPPTGGR------DTGPLAEATHFNLGEVV-GVLGGRHTCQAQGQCANVNCNVPKGYTYYSLTG--GPFQTKEKSHTFQSDA 3MYX Chain:A ((4-238)) QPTVLLLARADLSPVGTEFTTGPIDAHDPFDSGRRTAFVDEQGIAAGIVEFGTALSVEAYPYTEMLVMHRGSVTLTSGTDSVTLSTGESAVIGRGTQVRIDAQPESLWAFCASTQASGPDKSGITALDRLALLTPSSPPDPSIMISPLPQCRSNNLFEDTASTLRIGVWDSTPYERISRPHKIHELMNLIEGRVVLSLEN---GSSLTVNTGDTVFVAQGAPCKWTSTGYVRKFYAVT

General information: TITO was launched using: RESULT: Template: 3MYX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 31115 31.52 157.15 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 31.52 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_3MYX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MYX-query.scw PDB file : Tito_Scwrl_3MYX.pdb: