Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAMASLGALALLLLSSLSRCSAEACLEPQITPSYYTTSDAVISTETVFIVEISLTCKNRVQNMALYADVGGKQFPVTRGQDVGRYQVSWSLDHKSAHAGTYEVRFFDEESYSLLRKAQRNNEDISIIPPLFTVSVDHRGTWNGPWVSTEVLAAAIGLVIYYLAFSAKSHIQA 2YJL Chain:B ((30-52)) --------------------------------------------------------------RLRLYAVVQGRMQTIAE----RRYRVS------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2YJL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 33 -4266 -129.27 -185.48 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.50 3D Compatibility (PKB) : -129.27 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.679

(partial model without unconserved sides chains): PDB file : Tito_2YJL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YJL-query.scw PDB file : Tito_Scwrl_2YJL.pdb: