Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEV--------------EVSSLGVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRL----IN-LPLLYLGTLMVGASVAVINVLLPSLIQANQP-KKIGFLTTLYVTSMGIATALASYLAVPIT--QASSWKGLILLLTLLCLATFLVWLPNHRYNHRL----A-PQ-------------------------------TKQKSQIKVMRNKQVWAIIIFSGFQSLIFYTVM-TWLPTMSIH--AGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGVIGISMLFFP--------INNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL 4ZW9 Chain:A ((30-451)) --PALIFAITVATIGSFQFGYNTGVINAPEKIIKEFITKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQEPIYATIGAGVVNTIFTVVSLFLVERAG-RRT-LHMIGL-GGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELF-SQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGA-----------------------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1437 -156807 -109.12 -444.21 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -109.12 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: