Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MESRL--MKNKRYFFDTILIILLLISTIFCVSPVFIKLD-----ILGTPSH------AILTFVLAIPLFYILSQCL--------------HTLL-----LLVSSIFCKL---RPIYFYF-IFVIIIGARKYYRILFHQLM-------GFSPGIAVFYKESQT--------------------TKNLFKFYYFLYFTTLISYYFF------------FTFVYDKPLLLPLISFSIIIALVQKLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWK-LQSKSELPCVA--------------------------------LSYISLI-K-PYLSESVREQIDLLEVK-RFKKINHPI----------SLYGMLD--------------VIKLNLYLRHYNEKNKYESMLKKILEVRPDFVLIEQNIDDSLNS-SQPL-------SLSLAISEI-------------QLLLEVYMGIKHVSIRR 4ZH0 Chain:A ((27-527)) QDLSDRYESLNNLLNRYSTLNTLIKLSADPSAINAVRENLGASAKNLIGDKANSPAYQAVLLAINAAVGFWNVVGYVTQCGGNANGQKSISSKTIFNNEPGYRSTSITCSLNGHSPGYYGPMSIENFKKLNEAYQILQTALKRGLPALKENNGKVNVTYTYTCSGDGNNNCSSQVTGVNNQKDGTKTKIQTIDGKSVTTTISSKVVDSRADGNTTGVSYTEITNKLEGVPDSAQALLAQASTLINTINNACPYFHAPKFST--TTGKICGAFSEEISAIQKMITDAQELVNQTSVINEHEQTTPVGNNNGKPFNPFTDASFAQGMLANASAQAKMLNLAEQVGQAINPERLSGTFQNFVKGFLATCNNPSQGSAPGTVTTQTFASGCAYVGQTITNLKNSIAHFGTQEQQIQQAENIADTLVNFKSRYSELGNTYNSITTALSNIPNAQSLQNAVSKKNNPYSPQGIDTNYYLNQNSYNQIQTINQEL

General information: TITO was launched using: RESULT: Template: 4ZH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1454 -25598 -17.61 -78.04 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -17.61 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_4ZH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZH0-query.scw PDB file : Tito_Scwrl_4ZH0.pdb: