Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLGGGAISLVRNYIRNYGLGRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGDAITTIWANNDVNRRDPNLNALW 3SJA Chain:C ((1-61)) ---------------------------------------------------------------MNELSKKYLAKVKERHELKEFNNSISAQDNYAKWTKNNRKLDSLDKEINNLKDEIQSENHH

General information: TITO was launched using: RESULT: Template: 3SJA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 101 -627 -6.21 -10.28 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain C : 0.49 3D Compatibility (PKB) : -6.21 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.071

(partial model without unconserved sides chains): PDB file : Tito_3SJA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SJA-query.scw PDB file : Tito_Scwrl_3SJA.pdb: