Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSSAGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAGLEVTAIRDVTPVPHNGARPPKRRRV 4GKK Chain:K ((1-116)) ----------KRQVASGRAYIHASYNNTIVTITDPDGNPITWSSGGVIGYKGSRKGTPYAAQLAALDAAKKAMAYGMQSVDVIVRGTGAGREQAIRALQASGLQVKSIVDDTPVPHNGCRPKKKFR-

General information: TITO was launched using: RESULT: Template: 4GKK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 580 -43829 -75.57 -377.83 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain K : 0.85 3D Compatibility (PKB) : -75.57 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_4GKK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GKK-query.scw PDB file : Tito_Scwrl_4GKK.pdb: