Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------MEESKELNAVIDVIMLAG--TILLKSGSEIHRVEDTMIRIAHSQGIVDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDV----NQISRQLVGGQID-----LETAFKQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDA--------LGAGVATLFGFAFSLYVEKFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIAL-TNAVRDIMTNHINSGMSKMF-------ESLLIT----------------LALGAGTSVALVLMN--- 4FHL Chain:A ((2-392)) GSMTLSSNQYQLPLNVRPYTTTWCSQSPSCSNLLAIGHDTGITIYCASEESTGLTLQELFTIQTGLPTLHLSFSSSCSYSENSPVYSLFLACVCQDNTVRLIITKNETIITQHVLGGKSGHHNFVNDIDIADVYSADNRLAEQVIASVGDDCTLIIWRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGERNGNIRIFDWTLNLTELVKNPWLLTLNTLPLVNSSLANVRWIGSDGSGILAMCKSGAWLRWNLFKNLLPNVQGISLFPSLLGACPHPRYMDYFATAHSQHGLIQLINTYEKDSNSIPIQLGMPIVDFCWHQDGSHLAIATEGSVLLTRLMGFT

General information: TITO was launched using: RESULT: Template: 4FHL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1342 -64282 -47.90 -255.09 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -47.90 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.028

(partial model without unconserved sides chains): PDB file : Tito_4FHL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FHL-query.scw PDB file : Tito_Scwrl_4FHL.pdb: