Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGY------KLMNGDLILPEILEENLPIKVSFKPETKSTQ---LDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGG-IYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKA-ASLFKATAPITRNEL---IIEIWRAFFETPAEELL--YL--YKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK 2EWN Chain:A ((18-318)) ---------------EFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGS----VAVLPVIDSTNQYLLDRIGELKSGDAC----IAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVE--LTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWM-GGEISLRS--

General information: TITO was launched using: RESULT: Template: 2EWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1437 24688 17.18 87.23 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 17.18 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_2EWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EWN-query.scw PDB file : Tito_Scwrl_2EWN.pdb: