Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATWNSRIGAFFSLILLLLQLAS-SAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKSFS------ 2DOA Chain:A ((1-104)) --GSSGSSGVS----QRPFRDRVLHLLALRPYRKAELLLRLQKDGLTQADKDALDGLLQQVANMSAKDGTCTLQDCMYKDVQKDWP-GYSEGDQQLLKRVLVRKLSGPSSG

General information: TITO was launched using: RESULT: Template: 2DOA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 337 -37507 -111.30 -386.67 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -111.30 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_2DOA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DOA-query.scw PDB file : Tito_Scwrl_2DOA.pdb: