Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQG-PVGKIELFVDRPEGEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDEGHGETEDILAVIEHVRKL-HAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVGLRHYKTP-EIQGDILLIHGEQDDITLLSDAIEWAKP-Q-----KHPITILSGANHFFTGYLKQLRQIITRFIIMK 2I3D Chain:B ((23-231)) --------MPEVIFNGPAG-RLEGRYQPSKEKSAPIAIILHPHPQFGGTMNNQIVYQLFYLFQKRGFTTLRFNFRSIGRSQGEFDHGAGELSDAASALDWVQSLHPDSKSCWVAGYSFGAWIGMQLLMRRP---EIEGFMSIAPQPNTYDF----SFLAPCPSSGLIINGDADKVAPEKDVNGLVEKLKTQKGILITHRTLPGANHFFNGKVDELMGECEDYLDR-

General information: TITO was launched using: RESULT: Template: 2I3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1186 -12178 -10.27 -60.89 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -10.27 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_2I3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I3D-query.scw PDB file : Tito_Scwrl_2I3D.pdb: