Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MKMLAQLQNRFDQWVEQIVQYLDRLSVRERIMVVFTTIFVVVVIVG------YSLWKMHSLAEQQQKRLN-DLKDLMVWMQSNAVT-------------------------------MKPANELELDKSGKIQRVAQQQGLTVSS-----QQNGEQLQIVVTHQNYAILANF----LIQLAQMGL--SIQKM---EMVSSEGQIKLTATV---- 5IMU Chain:A ((4-220)) AGAVSARAAEQQRLQRIVDAVARQEPRISWAAGLRDDGTTTLLVTDLAGGWIPPHVRLPANVTLLEPTARRRDADVIDLLGAVVAVAAHESNTYVAEPGPDAPALTGDRSARSAIPKVDEFGPTLVEAVRRRDSLPRIAQAIALPAVRKTGVLENEAELLHGCITAVKESVLKAYPSHELTAVGDWMLLAAIEALIDEQDYLANYHLAWYAVTTRRG

General information: TITO was launched using: RESULT: Template: 5IMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 540 -8704 -16.12 -55.09 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -16.12 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_5IMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IMU-query.scw PDB file : Tito_Scwrl_5IMU.pdb: