Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFLPVILSLALITNASYANPF-DPKPASGQVEIPNIGSGIGLLDQQKEKFIGEKVFREVHKQMPVIQDVWLEDQFFQVFSNILSETQLGQPIALVVIKDPQINAFAVPGGLFALNTGLISSARNIDEIAGVMAHEIAHVSQRHFSRSEEAFKGQTLLSLAGLLAGVALAAQAGGDAGAAVMLGTQAALLDKQLTYSRNQEREADRIGM---QYMYAAGYNPQSMADYFETMHRATSRVSFLPDFWLTHPLTSERMSEARLRANQMPKVKSRIYDVDFEILKWYTMVVAGEATENQLQSLASQKNLAGLLALSAFYLKQGDYTQAQATLEQAKSSGKPLVALIQTDIYLGQNKINQAYNSIAPLQMTMPENKAFSYKLAEVLLRQGK-YAQVQTLVQRFINKNARDIQGWQLLQQAANLDKNSPLRAVNVLRYRAEAQYWSGSEEDAIKSMLHAQRLAKGNQAMSARIDSRLKQMQDERRMKI 5I0P Chain:A ((13-366)) --------------EHQLDYPFADTLPAAGDT--FEVAPGVRWLRMP------------LPFSLDHINLWLLRDEI---------DGQAGWTIVDCGISSEAIRTHWEQ--IFDRHLDGLPVLRVL----------VTHCHPDHF--------------------GLANWLCEGGDQG-----------------------RWNVRLWMTLGEYMFGCLMAAGAAADHFARHGL------YYSDLVPAVPPRYRRLREGD----------------AVKIGARTWRVVTGYGHSPEHCALHSEAD--GVLISGDMVLPR---ISTNVSVFDLEPEANPLALYLES---------LGRYETMAPDTLVLP--------------SHGKPFRGVRTRIAQLRAHHDARLEEVRVACAE------KPMSAAGIVPIMFT--------FALGEALAHLNLLWLAGELVREHGDDGVIRYARA-----

General information: TITO was launched using: RESULT: Template: 5I0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1775 -15219 -8.57 -48.01 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -8.57 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_5I0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I0P-query.scw PDB file : Tito_Scwrl_5I0P.pdb: