Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPPERPENDKTQKAKDNITGQISKYEKIVSYEGREAANNGGLRKALEDFARTGEKVGTKNPSNHIQKARDILRGLDNQEKLLQKSTINSQDKAVLQQRIQSLRNEIEPSLKRAITKQNELN 1KK2 Chain:A ((160-252)) -----------RQIKEFIEGTVAENAPIIPISALHGANIDVLVKAIEDFIPTPKRDPNKPPKMLVLRSFDVNKPG-----LVG----GVLDGSIVQGKLK-VGDEIE--------------

General information: TITO was launched using: RESULT: Template: 1KK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 136 -2483 -18.25 -28.87 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -18.25 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_1KK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KK2-query.scw PDB file : Tito_Scwrl_1KK2.pdb: