Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKFVDDHHLKRKVGTLEMVVAMLLSGSIGLFVIKSGQSPINIVFFRCLISALCLIPICYFYGHFRKVYFGKKELFLMVTSGLLIIFNWVLLFAAFPKTSISLATIVYHVNPFVILFLGALVFHEKLNKNDVLWTIIAFIGLIVIIGLGSASVN----SNELVGLGLVLIATTLYSISVLITKKLSNTPPLLIVFIQTLSGAIVMAPFISVFENPPIGQQWLFVVGLGVLHTAFLYYLMYSAIKKIP---------------LNNIAILSFIYPISTIVI---------DYFFFDHVLTSTQVLGAGLILLGVLGVKLHWNIFAVKKVGIRN 5MUW Chain:A ((2-276)) -PIVVTQAHIDRVGIAADLLDA----------------SPVSL-----------------------QVLGRPTAIN---TVVIKTYIAAVMELASKQGGSLAGVDI----RPSVLLKDTAIFTADVESDVDVLDT-----GIYSVPGLARKPVTHRWPSEGIYSGVTALMGATGSGKSITLNEKL--RPDVLIRWGEVAEAYDELDTAVHI---STLDEMLIVCIGLGALGFNVAVDSVRPLLFRLKGAASAGGIVAVFYSLLTDISNLFTQYDCSVVMVVNPMVDAEKIEYVFGQVMASTV----GAILCA--DGNVSRTMFRTNKGRIFN

General information: TITO was launched using: RESULT: Template: 5MUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1210 -175883 -145.36 -729.80 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -145.36 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.207

(partial model without unconserved sides chains): PDB file : Tito_5MUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MUW-query.scw PDB file : Tito_Scwrl_5MUW.pdb: