Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MHEIT-LHE-VRQLIASLRTVYAAQFNKQFPTSGESAIPLSVVEQIALKTLVGVQQNQFNNALARLLTAGGRFMPSFAEFRTWCIGESWMSPEEAWSRACKFTTDRTVVITQITKYA-LDEVMYLIEAGQMRAAQDNFFGTYNVMVAKAQLKGR-QQEFYTPPLQLEHKEPEHTP-VSNDEVQKQLKSL-MERLKINGRKPAPVQKLQAKEKEPELAKELGPDPFDNPHEYAEMCRREGMPIPR---NILQLIEGANV 2P7I Chain:A ((23-247)) NFDFDVMHPFMVRAFTPFFRPGNLLELG---SFKGDFTSRLQEHFN----DITCVEASEEAISHAQGRLKDG--------------ITYIHSRFEDAQLPRRY--DNIVLTHVLEHIDDPVALLKRINDDWLAEGGRLFLVCPNANAVSRQIAVKMGIISHNSAVTEAEFAHGHRCTYALDTLERDASRAGLQVTYRSGIFFKALANFQWDQILQTD---------ILSKEYLDGCYQLGQQYPDLCASIFLLCEKG--

General information: TITO was launched using: RESULT: Template: 2P7I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1002 14982 14.95 69.68 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 14.95 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_2P7I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P7I-query.scw PDB file : Tito_Scwrl_2P7I.pdb: