Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFDRNTPIAFLGIGLMGNRMASHLIQAGFQV--AVWNRTASACEELIDIGAHALDLSNIGQY-PLILTCLADDKAVQAVF---DQIQTNLKAGQVIVDFSSLSVAATKALAQAAALQDVIWIDSPVSGGTAGAEQGTLVIFAGGDAQTIEALSPVYNVLSQRVTRMGD-TGTGQATKICNQLIVAANSALIAEAVALADRAGVDTTLLAPALAGGFADSKPFQI-LAPRMATHTFEPVQWKVQTLSKDLNNAVTLANNVNLDIPVAQKALLQLQTHQKNSFAEKDLATMIQVVEQ 5JE8 Chain:A ((7-295)) -----MKKIGFIGLGNMGLPMSKNLVKSGYTVYGVDLNKEAEASFEK-EGGIIGLSISKLAETCDVVFTSLPSPRAVEAVYFGAEGLFENGHSNVVFIDTSTVSPQLNKQLEEAAKEKKVDFLAAPVSGGVIGAENRTLTFMVGGSKDVYEKTESIMGVLGANIFHVSEQIDSGTTVKLINNLLIGFYTAGVSEALTLAKKNNMDLDKMFDILNVSYGQSRIYERNYKSFIAPENYEP-GFTVNLLKKDLGFAVDLAKESELHLPVSEMLLNVYDEASQAGYGENDMAALYKKVSE

General information: TITO was launched using: RESULT: Template: 5JE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1477 -639 -0.43 -2.27 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -0.43 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_5JE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JE8-query.scw PDB file : Tito_Scwrl_5JE8.pdb: