Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYFMSFKDELAAQVAQRRTFAIISHPDAGKTTMTEKLLLWGKAIQVAGMVKSRKSDRAATSDWMEMEKERGISITTSVMQFPYKGHTINLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEERTIKLMEVCRMRDTPIISFVNKMDREIREPLELLDEIENVLNIRCVPITWPLGMGRDFAGVYNILEDKLYVYKAGFGSTITDIEVRDGY-DHA-DIREKVGELAWASFEESLELVQMANEPLDRGLFLQG--------KQTPVLFGTALGNFGVDHVLDAFMHWAPEP------KAHPTQERVVEAKEE---GFSGFVFKIQANMDPKHRDRIAFMRICSGKYEKGLKMNHVRISKEVRISDALTFLAGEREHLEEAWPGD---IIGLHNHGTIQIGDTFTSGENLHFTGIPHFAPEMFRRVRLKDPLKSKQ--LQKGLKELSEEGAT-QVFMPQISNDLIVGAVGVLQFDVVAYRLKEEYKVDCVYEPVSVNTVRWIHCDDEKILNEFKKKAHDQLSIDGGGHLTYLAPSRVNLQIMQERWPDIEFRSTREH 2J7K Chain:A ((13-474)) -----------------RNIGIAAHIDAGKTTTTERILYYTGRIH----------------------------ITAAVTTCFWKDHRINIIDAPGHVDFTIEVERSMRVLDGAIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQAREYHEKLVEVA-ADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPLAALAFKIMA--DP-YVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVARLLRMHANHREEVEELKAGDLGAVVGLKETIT---GDTLVGEDAPRVILESIEVPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISGMGELHLEIIVDRLKREFKVDA--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2J7K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2141 -46928 -21.92 -113.63 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -21.92 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_2J7K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J7K-query.scw PDB file : Tito_Scwrl_2J7K.pdb: