Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELTPYHEDQELDNNTSDNIHFRDILEQHISRRSLITKAASGAVALTLASTLTGCNDNDDDSGSNNGGTTPVDPNKKPEKLTFTPVAKNLNDIVTVPEGYEANVIYALGDSINPRVGDWDDNNIPSGPSFQFRSGDCHDGMHYFGLNTSTNRFDETVSAQGLLVMNHEYINQTFLHPKGPTKVDGRRPEDEVIRETNANGVSVIHIKKDPTTQKVEIIKNSIFNRRITASTVMEFAGAAAGSSLLATRFSPAGRQTRGTHNNCGNGYTPWGTYLTTEENFIGYFQRSGSDEYARTDAEKITLKRYGLGVKKDEPYLYEKDEKGAPKKDKDGKIIYLKDKNGELIPNVDEQGRQIYLGTSSRYGWETAIGQVESQDLYDRWNADVKAAQATQDYRNGPNTFGWMVEIDPFDGRQNPVKRTSLGRFAHEDSACRAVVGQPLAFYMGDDSRGEYIYKFVSTAVWDTKDINGGYTAGDKYMNAGKLYVAKFNN--------DGSGQWIELAYGKNGLNESNTTYPFKSQADVVTFARLAADSVGATKMDRPEWCTINPLNGEIYVTLTNNSNRGKD-YATDAANPRNYTDLYAGTKEQKGNVNGHIIRFKETDDKTTAETFKWDIYLFGAEAAMASNINLSG---LTDNNDFSSPDGMWFDPRGVLWIETDDGAYTDVTNCMMLAALPGQIGDGGTATTSNGQQTITGAKVTDATLRRFLVGPKQCEITGIAMTPDYKAIFINVQHPGEDSPSYAKPESNWPATQKDPSNKTARPRSATVVITRKDGGVIAG 4AMF Chain:B ((7-581)) ---------------------------------------------------------------------------------LGFDSIPAATTDTISLPKGYKSSVLISWGQPLHKNGPAFDPSGNGTAAAQEVQFGDNNDGMSLFEFPGEKNR--------ALMAINNEYTNYRYLYPHGGMP----QSAEDVRKALACEGVSVIEVQR--KNGQWQFVQGSRYNRRIHGNSPLRISGPAAGHELMKTSADKHGKKVLGTFQNCANGKTPWGTYLTCEENFTDCFGSSNAQQ--QFDPAQ---KRYGVSAASREINWHPFD----PRFD------MAKNPN-EL----------------NRHGWVVEIDPFDPQ--------------------------------------STPVKRTALGRFKHENAALAETDDGRAVVYMGDDERGEFIYKFVSRDKINHRNAK----ANRDILDHGTLYVARFDAGDGNPDHPKGQGQWIELTHGKNGIDASSG---FADQAEVLIHARLAASVVGATRMDRPEWIVVSPKDGQVYCTLTNNAKRGEDGQPVGGPNPR------------EKNVYGQILRWRTDRDDHASKTFAWDLFVVAGNPSVHAGTPKGGSSNITPQNMFNSPDGLGFDKAGRLWILTDG----DSSNAGDFAGM--------------GNNQMLCADPATGEIRRFMVGPIGCEVTGISFSPDQKTLFVGIQHPGENG------GSTFP--EHLPNGK---PRSSVMAITREDGGIVG-

General information: TITO was launched using: RESULT: Template: 4AMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3792 73013 19.25 129.69 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 19.25 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_4AMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AMF-query.scw PDB file : Tito_Scwrl_4AMF.pdb: