Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQVKIVPVTAFAQNCSLVWDSETKEAVLIDAGGD------------AAVLKKEVEALGLKVKALWLTHGHLDHAGAVGELAKEWSVPV-VGPHKEDQFWLDMIQEVSARYGFPIP----QPVKVDQWLEGGEVLKLGEDEFEVRFAPGHTPGHVMFY--------NKNHGLLWTGDVLFKGSIG--RTDFPRGNHEQLIESIQRECFSLPDETQFISGHGPMSTIGYEKQFNPFVAGKAG 4EFZ Chain:A ((21-219)) -----------------LLFDSGSGECALIDSVLDYDPKSGRTRTASADQLIARVAALGARVRWLLETHVHADHLSAAPYLKTRVGGEIAIGRH------VTRVQDVFGKLFNAGPAFAHDGSQFDRLLDDGDTLALGALSIRAMHTPGHTPACMTYVVTEAHAAHDARDAAAFVGDTLFMPDYGTARCDFPGGDARSLYRSI-RKVLSLPPATRLYMCH---------------------

General information: TITO was launched using: RESULT: Template: 4EFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 785 -23165 -29.51 -137.07 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -29.51 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_4EFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EFZ-query.scw PDB file : Tito_Scwrl_4EFZ.pdb: