Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKMKSPILRLRRLRRTKAIRGLLCENNFSVEDLVQPIFIEESITQPIPISTMPGIYRLPEQDIEQEVKALYALGIRCVMPFGIS--HHKDSMGSDTWSDTGLLVRMIKAIKSSCPEMIVTPDICFCEYTTHGHCGVMIHD-QISNDLTVENLIKQSLAAAKAGADMLAPSGMMDGQVGAIREALDQAGYHNVLIMAHAIKFASALYGPFRVAVD--SNL-VGDRHSYQLDYANTRQALREAALDESEGADLLIVKPGMFYLDILSQLRAQTKLPLAAYQVGGEYAAIKFAAIAKALDERKTVIESITAFKRAGADVIITYFAKDIAIWHNEF 1B4K Chain:A ((11-330)) ------PYTRLRRNRRDDFSRRLVRENVLTVDDLILPVFVLDGVNQRESIPSMPGVERLSIDQLLIEAEEWVALGIPALALFPVTPVEKKSLDAAEAYNPEGIAQRATRALRERFPELGIITDVALDPFTTHGQDGILDDDGYVLNDVSIDVLVRQALSHAEAGAQVVAPSDMMDGRIGAIREALESAGHTNVRIMAYSAKYASAYYGPFRDAVGSASNLGKGNKATYQMDPANSDEALHEVAADLAEGADMVMVKPGMPYLDIVRRVKDEFRAPTFVYQVSGEY-AMHMGAIQNGWLAESVILESLTAFKRAGADGILTYFAKQAA------

General information: TITO was launched using: RESULT: Template: 1B4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1685 -28305 -16.80 -90.14 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -16.80 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_1B4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B4K-query.scw PDB file : Tito_Scwrl_1B4K.pdb: