Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVTAIVKPFKLDDVREALSDIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEIAISDEMVDAVIESITRVASTGKIGDGKIFLTNLEQVIRIRTGETGPDAV 2PII Chain:A ((1-112)) MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTELYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRIRTGEEDDAAI

General information: TITO was launched using: RESULT: Template: 2PII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 387 -50058 -129.35 -446.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -129.35 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2PII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PII-query.scw PDB file : Tito_Scwrl_2PII.pdb: