Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKAQKLTLAVLISAAIISSAQASEQSEAKGFVEDANGSILFRTGYLTRDKKQGAKDTSSV-AQSAIVSIESGFTPGIVGFGVGVVGDGSFKI-GENKNTGNQMIPKHNDGSAYDHWARGGGSVKARFSNTTVRYGTQVLDLPVLASNTGRMVPEYFTGTLLTSHEIKNLEVVAGKFTKDQM---SDQINTDADASGRGLDRAIVWGAKYKFNDNLNASYYGLDSKNALERHYANVNFKQPLANDSSLTYDFSGYHTKFDANAHTYSATGTVAPNYAADGIADEEKTNNIWAISGTYATGPHSVMLAYQQNTGNVGYDYGQNADGFQSIYLPNSYMSDFIGNHEKSAQIQYNVDFGKLGVLPGLNWTTAFVYGWDIKVRNVTDDAQEREFFNQVKYTVQSGFAKDASLRIRNSYYRASDAYQGAYIGDTNEWRIFLDIPVKLF 2Y0K Chain:A ((8-390)) -------------------------------LVEDSHASLELRNFYFNRDFR-GARDNADEWAQGFLLRLESGFSEGTVGFGVDAIGLLGFKLD-------------------QDDYAKLGLTAKARVSNSLLKVGALHFKSPLVSANDTRLLPELFRGALLDVQEIDGLTLRGAHLDRNKLNSSSDYQVFSANRIGGRSDAFDFAGGDYRLTPALTASLHQGRLKDIYRQTFAGLVHTLDLG--RSLKSDLRFARASEDGGFR--------------------ELDNRAFGALFSLRLGAHAVAAGYQRISGDDPYPYIAGSDPYLVNFIQ---IGDFGNVDERSWQLRYDYDFGALG-LPGLSFMSRYVSG-DNVARGAANDGKEWERNTDLGYVVQSGPLKNLGVKWRNATVRSN------FANDLDENRLILSYSLALW

General information: TITO was launched using: RESULT: Template: 2Y0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2006 30846 15.38 87.14 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 15.38 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_2Y0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y0K-query.scw PDB file : Tito_Scwrl_2Y0K.pdb: