Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDTRENWTSRSGFIIAAVGSAVGLGNIWRFPYVAYENGGGAFLIPYLLALITAGLPLLFLDYAVG-HRSTGSPPKAYR--ALFKGGETLGWWQVCVCIIIGLYYASVLTWA---------GSYVYFSIGQMWGS------DPEGF------FFNTY-LQTTKATGFDLQFVGHLFWPIVGIWALTLIILYGGVKKGVELSNKI--FMPLL-FILFTILVIQSLRLPGAVQGLNAFFTPNWSAMMDYKVWLAAYGHTFFSLSVGFGIMVTYASYLKPKTNLTGSGLIVGFANASTEILAGIGIFAALGFMAHAAGTEVKDVVSGGIGLAFIAFPKIISSLGSGADLFGLLFFSSLFVAGISSMVSILEVPIAAMQDKLKWGRKKAVTIIGGGSALVSIILFSSVNA--IKLVDIVDHFINNIGIIGGALLSIISVAWF--KRSALKELRDHVNRISTIQLGKGWDFTLTVITSLILLTTLCMTVFNLIKNGYDTYS----MSLQGVFGW----GSVIFCAVVAIVLSKMKDR 4M48 Chain:A ((6-514)) --DERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLAVGGIPLFYMELALGQHNRKGAITCWGRLVPLFKG---IGYAVVLIAFYVDFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFESQGFQSAASEYFNRYILELNRSEG--IHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAALLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWADAATQVFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATM-PASTFWALIFFMMLATLGLDSSFGGSEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGTNRFSEDIRDMIG----FPPGRYWQVCWRFVAPIFLLF---ITVYLLIGYEPLTYADYVYPSWANALGWCIAGSSVVMIPAVAI--------

General information: TITO was launched using: RESULT: Template: 4M48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2605 -342009 -131.29 -729.23 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -131.29 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_4M48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M48-query.scw PDB file : Tito_Scwrl_4M48.pdb: