Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVVFSGAGMSAESGISTFRDSNGLWENYDIQQVATPEAWERNPALVQRFYNERRKNILEAQPNEAHQYIAKLQDHYDVQ-----VITQNIDDPHERAGSQNVLHLHGNIRLAKS------------------SGPDA-QYTTQFYEVNGWKL-DLEQDFCPNGYPLRPHVVWFGEAV-PA-YEEAIRLVQSADIFIVIGSTLSVYPVAALVHEIPHYSKAYYIAPQADHSRVPPQYKLLNMTATEGMHELFNQLTS 4G1C Chain:A ((18-229)) ---IVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGC--GGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQV------------------------------------------

General information: TITO was launched using: RESULT: Template: 4G1C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 846 -3548 -4.19 -19.18 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -4.19 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_4G1C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1C-query.scw PDB file : Tito_Scwrl_4G1C.pdb: