Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKRLIGLFSPDLAIDLGTANTLIYAPGRGIILNEPTVVAI-RHSGSQKIVAAVGLDAKQMLGRTPANISAIRPMKDGVIADFEVTETMLNQFIGKVHEKRLFPPAPRVVVCVPCKSTLVERRAIREAVFNASARDVRLIEEPMAAAIGAGMPVEQACGSMVVDVGGGTTEIAIISLQGCVY-----ADSLRIGGDVFDEQIINYV----RKAHGCVIGETTAEIIKKEVGMAVSDGTTLEIEVRGRNLAEGVPRAITVTSDEITQAISDPLQSIVSAVKSALEQTPPELSSDIAERGIVLTGGGALLRNLDKLLAQETGLPVVVAEDPLTCVTRGGGKVLEFFDDPNHDMLFVG 2V7Y Chain:A ((55-218)) -----------------------------RQAITNPNTIISIKRHMGTD---YKVEIEGKQY---TPQEISAI-----------------ILQYLKSYAEDYLGEPVTRAVITVPAYFNDAQRQATKDAGRIAGLEVERIINEPTAAALAYGLDKEEDQTILVYDLGGGTFDVSILELGDGVFEVKATAGDNHLGGDDFDQVIIDYLVNQFKQEHG--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2V7Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 555 -618 -1.11 -4.01 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -1.11 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_2V7Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V7Y-query.scw PDB file : Tito_Scwrl_2V7Y.pdb: