Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIVCLDLEGVLVP-EIWINFAKKTGIKE-----LEATTRDIPDYDVLMTQRLNILKQHGLGLKDIQEVIAEMGPLPGAKEFVEWVSNH-FQLVILSDTFYEFAHPLMKQLGWPTIFCHKLETDENGMITAYKLRQPDQKRESVKALHGLNFR-------VIAAGDSYNDTTMLGEADHGFLFDAPANVIAEFPQFPAINGYDALKEAIRSVSQRNIPA 5IT4 Chain:A ((178-358)) -RLIVFDVDSTLVQGEVIEMLAAKAGAEGQVAAITDAAMRGELDFAQSLQQRVATLA--GLPATVIDEVAGQLELMPGARTTLRTLRRLGYACGVVSGGFRRIIEPLAEELMLDYVAANELEI-VDGTLTGRVVGPIIDRAGKATALREFAQRAGVPMAQTVAVGNGANDIDMLAAAGLGIAFNA----------------------------------

General information: TITO was launched using: RESULT: Template: 5IT4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 818 -5117 -6.26 -30.64 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -6.26 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_5IT4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IT4-query.scw PDB file : Tito_Scwrl_5IT4.pdb: