Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLISLPKQILQCGQLQLDLSQPH-VMGILNVTPDSFSDGGKHNQLDQAVDHALSMIEQGATIIDIGGESTRPGASEVAVEEEVRRVVPVVEALSHH-NVILSIDTSQPEVIRAAKAAGAHIWNDV-RALTGPNALKTAVELDIPVVIMHMRGEPTTMNQLDQYTDVTLDVMQELQQRIDEALAAGVKKHNIIVDPGFGFAKNAQQNLKLLKEFWKLNELGYPILSGLSRKRFIGEALQGAPADQRAVGSVTGHLLSIQQGASIVRAHDVKEMHEAILVWKAMQQA 3H22 Chain:A ((27-291)) ----------LRCGEYTLNLNEKTLIMGILNVTPDSF--GGSYNEVDAAVRHAKEMRDEGAHIIDIGG----------SVEEEIKRVVPMIQAVSKEVKLPISIDTYKAEVAKQAIEAGAHIINDIWGAKAEPKIAEVAAHYDVPIILMHNR---DNMN----YRNLMADMIADLYDSIKIAKDAGVRDENIILDPGIGFAKTPEQNLEAMRNLEQLNVLGYPVLLGTSRKSFIGHVLD-LPVEERLEGTGATVCLGIEKGCEFVRVHDVKEMSRMAKMMDAM---

General information: TITO was launched using: RESULT: Template: 3H22.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1412 -6661 -4.72 -26.64 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -4.72 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_3H22.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H22-query.scw PDB file : Tito_Scwrl_3H22.pdb: