Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTQPMIEKLIEAHLDFLDEQFAQTQVIQQEFEQFYHWLGSRQLQHLWTFEQVQQLIQKQILDTPASDFLIEQIAEHIRFALIHPTNDTATVEDVIPVLTIDRIAQYVASKEEHRKKLIKTIVNNPAFSALLTQLIQQTMHDYLDESMTKRVPGVGRFMKMGKSVLETVTDSNLDDTINHYLQKNILKLSQMSKRVLNQHFDNDKLYHFQANVWHKVKTSPLSVLKNYIEVQDLTRTVGLGHEIWDHIRQTDYLKQQVHDGIYTWYVRNQERNFDLLLRDLNIDENLVKHELTELLVPVLQQLVTTGHLRRRARVYLEKFYYSEKALEILNNKGA 4AJU Chain:A ((276-330)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TMLVVLMEKM-DHLALEDYLTT---DLVETWLRNMKTRNMEVFFPKFKLDQKYEMHEL-------------------------------------------

General information: TITO was launched using: RESULT: Template: 4AJU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 33 -7350 -222.73 -136.11 target 2D structure prediction score : 0.04 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -222.73 2D Compatibility (Sec. Struct. Predict.) : 0.04 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_4AJU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AJU-query.scw PDB file : Tito_Scwrl_4AJU.pdb: