Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLQGCLIVIKTDVIFDESNIIIGLFTIKDDHGIIQISTNGNQVNWD---EWVDEAGGHSLEKAIIYCLNRKPPEDATHYKETKKQNSYRYYKKVEEDW---FIYVDWRYPLGWQPTGMFDDITKIKPLPQFG 5FFR Chain:A ((40-102)) -------------------------------------STNGQSVNFDTIKE-CTRAGGN-------IAVPRTPEENEAIASIAKKYNNYVYLGMIEDQTPGDFHYLD--------------------------

General information: TITO was launched using: RESULT: Template: 5FFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 112 8445 75.40 150.79 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 75.40 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_5FFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FFR-query.scw PDB file : Tito_Scwrl_5FFR.pdb: